Matrigengel Matrix基质胶

Matrigengel Matrix基质胶

基质胶实验应用—2D，3D细胞培养

传统细胞培养是在单层中生长，细胞是扁平并可以在水平面上自由粘附和扩散，但不支持在垂直方向上扩散；2D表面上培养的细胞具有强烈的顶端-基底极性，这种极性与某些细胞类型相关，例如上皮细胞；3D细胞培养即三维细胞培养，是指具有三维结构的不同材料载体与各种细胞在体外共培养，使细胞在载体的三维立体空间结构中迁移，生长，形成三维的细胞-载体复合体，主要用于细胞球，类器官等培养。

作为疾病验证模型，相比于传统2D疾病模型，以3D为基础培养的类器官在阐明疾病的发展，稳态性和发病机理等与体内环境更加接近，在多领域将发挥重要，诸如细胞治疗，药物研发，基因工程，免疫研究，组织再生等领域。基质胶作为类器官3D培养的重要载体，在其中发挥着举足轻重的作用。

2D和3D培养对比

基质胶是从富含胞外基质蛋白EHS小鼠肿瘤中提取中的可溶性基底膜制备物，其主要成分由层粘连蛋白，IV型胶原，硫酸乙酰肝素蛋白聚糖（HSPG）和巢蛋白组成，还包含生长因子如TGF-beta，EGF，IGF，FGF，组织纤溶酶原激活物和EHS肿瘤自身含有的其他生长因子，为组织和细胞提供支撑，抗拉强度和支架支撑，也可以作为细胞粘附和运动的三维亚结构以及生长因子，趋化因子和细胞因子的储存库，同时可作为细胞形态发生和分化的信号等。

针对特定应用的不同版本基质胶

基质胶产品列表

基质胶产品应用数据

干细胞培养

Matrigengel (Cat. No. 354277) 1:100 dilution coating the plates, supports stable passage of EPSCs (extended pluripotent stem cells) from P1 to P4.

类器官培养

Human tumor organoids (colorectal cancer organoids, Human tumor organoids (breast cancer organoids,

 gastric cancer organoids) can grow well in  hepatocellular carcinoma organoids) can

greater than 70% Matrigengel (Cat. No.356255). grow well in greater than 70% Matrigengel (Cat. No. 356255).

Mouse intestinal organoid, liver ductal organoid, airway organoid can also grow well in greater than 70% Matrigengel (Cat. No. 356255).

血管生成试验

The complete medium and HUVECs suspension (2×10⁴ /well in 96 wells) The complete medium and HUVECs suspension (2×10⁴ /well in 96 wells)

can form the excellent tubular structure on 100% and 67% Matrigengel can form the excellent tubular structure on 100% and 67% Matrigengel

Matrix (Cat. No. 354248) at 4h, which can be quickly performed for Matrix (Cat. No. 3554248) at 4h, which can be quickly performed for

subsequent staining and angiogenesis analysis. subsequent staining and angiogenesis analysis.

VEGF and Heparin could promote the in vivo angiogenesis through in vivo Matrigengel plug assay. (Cat. No. 356242).

肿瘤细胞侵袭

The invasion effect of 5×10⁴ SGC-7901 was detected for 24 hours at different concentrations of Matrigengel (Cat. No. 354234) and it was clear that the number of successfully invaded cells decreased significantly as the concentration of matrigel increased.

The invasion effect of 5×10⁴ SGC-7901 was detected for 24 hours at different concentrations of Matrigengel (Cat. No. 354233) and it was clear that the number of successfully invaded cells decreased significantly as the concentration of matrigel increased.

体内成瘤实验

Matrigengel (Cat. No. 356248) significantly promotes the proliferation of tumor cells under the skin in nude mice.